The Chlamydomonas Flagellar Proteome Project
ChlamyFPv5 (gene name | annotation): FAP5 | "FAP5, Flagellar Associated Protein 5; c-terminus is Similar to Nephrocystin 3"
Aliases: FAP5; Nphp3
Structure:
References:
CrFP Total Peptides = 24 (0 Axonemal Peptides | 22 M+M Peptides | 1 KCl Peptides | 1 Tergitol Peptides)
Wang Peptides (Wang et al. Pan 2017) = 22 (enriched 1.29 fold by ciliary shortening)
Phytozome (gene name | defline): | Flagellar Associated Protein
O. Vallon Gene Name | Defline: FAP5 | Flagellar Associated Protein 5
O. Vallon Description: Conserved uncharacterized Flagellar Associated Protein; found in the flagellar proteome [PMID: 15998802]. Possible kinesin; C-terminus is similar to Nephrocystin 3
O. Vallon Synonyms: g16844.t1#Cre01.g071200.t1.1#FAP5
Steve King Dynein Anotation: |
Yuqing Hou's Human Homologs: KLC2, NP_001128246.1, kinesin light chain 2 isoform 2 [Homo sapiens] (Blast E value: 2.00E-10 | Reprocal Best Match? No)
Albee et al. Dutcher 2013 Human Homolog: NPHP3, nephronophthisis 3 (adolescent)
Lipid Modifications (N-Myristoylation: MGXXXS/T, S-Geranylgeranylation: CAAX , S-Geranylgeranylation: CC/CXC, S-Farnesylation: CAAX predicted by GPS-Lipid): N-Myristoylation: MGXXXS/T
Fold Upregulated Post Deflagellation (Albee et al. Dutcher 2013) at 3 min: 2.82; at 10 min: 9.23; at 30 min: 11.49; at 60 min: 3
Importance of XAP5 for expression (Li et al. Hu 2018) Wild Type: 128.72 FPKM | XAP Mutant: 24.46 FPKM | Fold Change: -2.4
CLIP Library: Cre16.g687966
Phytozome Transcript: Cre16.g687966.t1.1
PhosphoPeptides
Protein Reference Source Peptide pLOGO
(Pan et al. Hippler 2011)Purified Flagella GPAEFELPERDVEPLAPADSVR
ChlamyFP Peptides
Fraction Sequence
(click on peptide to see other models containing this peptide)
T29LQGLQDAVER
C14AAAGAAAAAGGQQTATEAR
B30WTEQDLASK
B11GPAEFELPERDVEPLAPADSVR
B10QQEAQADDTSLTMR
B10RQQEAQADDTSLTMR
B10STLAADVGWR
B10AKEAEALFK
B10KIDASPTK
B10VALTAPGAR
B10NAPQTQEWLK
B09LSAPDTAAAAAGVK
B09HLGDYHPDAANVLAGLADNVKGEGR
B09GAVAEPLYR
B09VALGPLAAAAPGEAGHTAK
B08LPFPGNVAAQLLVR
B08GAGSAGEVDAR
B08ARPDAMDGAAAAR
B08FSQAEPLYR
B08AQGMTFDAEDTIEELTAGK
B08AAVEGPGSVR
B08DAKAAEADAR
B08TPLLPADGK
B07LGEGGAGAVPAGDVAAIAELVSSDSAR