The Chlamydomonas Flagellar Proteome Project
ChlamyFPv5 (gene name | annotation): TUB1 | "TUB1, beta-1 tubulin"
Aliases: TUB1
Structure: Axoneme; Transition Zone
References: (Youngblom et al. Silflow 1984); Transition Zone Protein (61 spectral counts [Diener et al. Rosenbaum 2015]); Ubiquitinated (Wang et al. Huang 2019)
CrFP Total Peptides = 28 (4 Axonemal Peptides | 5 M+M Peptides | 14 KCl Peptides | 5 Tergitol Peptides)
Wang Peptides (Wang et al. Pan 2017) = 23 (enriched 1 fold by ciliary shortening)
Jordan Peptides (Jordan et al. Pigino 2018) = Wild Type M+M: 26 | Dhc1b-3 M+M: 29 | Ift139-2 M+M: 24 (Total Unique Peptides)
Picariello Peptides (Picariello et al. Witman 2019) = Wild Type M+M: 28 | Ift140 ΔN-terminal WD repeat M+M: 33 (Total Unique Peptides)
Phytozome (gene name | defline): TUB1 | Beta tubulin 1
Annotation v5.6 Symbol | Defline: TUB1 | beta-1 tubulin
Annotation v5.6 Description: GI:167458; major constituent of microtubules [PMID: 3460413, PMID: 6098820]; it binds one mole of GTP that is hydrolyzed and alters dynamic instability of the microtubule cytoskeleton
Annotation v5.6 Synonyms: g13727.t1#RSP19
Steve King Dynein Anotation: |
Yuqing Hou's Human Homologs: TUBB4B, , (Blast E value: | Reprocal Best Match? )
Albee et al. Dutcher 2013 Human Homolog: TUBB4B, tubulin beta-4B chain
Lipid Modifications (N-Myristoylation: MGXXXS/T, S-Geranylgeranylation: CAAX , S-Geranylgeranylation: CC/CXC, S-Farnesylation: CAAX predicted by GPS-Lipid):
Fold Upregulated Post Deflagellation (Albee et al. Dutcher 2013) at 3 min: 5.12; at 10 min: 6.96; at 30 min: 7.52; at 60 min: 5.17
Importance of XAP5 for expression (Li et al. Hu 2018) Wild Type: 7304.14 FPKM | XAP Mutant: 1476.53 FPKM | Fold Change: -2.31
CLIP Library: Cre12.g542250
Phytozome Transcript: Cre12.g542250.t1.1
PhosphoPeptides
Protein Reference Source Peptide pLOGO
(Pan et al. Hippler 2011)Purified Flagella FWEVVSDEHGIDPTGTYHGDSDLQLER
(Pan et al. Hippler 2011)Purified Flagella FWEVVSDEHGIDPTGTYHGDSDLQLER
(Pan et al. Hippler 2011)Purified Flagella FWEVVSDEHGIDPTGTYHGDSDLQLER
(Pan et al. Hippler 2011)Purified Flagella FWEVVSDEHGIDPTGTYHGDSDLQLER
(Pan et al. Hippler 2011)Purified Flagella FWEVVSDEHGIDPTGTYHGDSDLQLER
(Pan et al. Hippler 2011)Purified Flagella FWEVVSDEHGIDPTGTYHGDSDLQLER
(Pan et al. Hippler 2011)Purified Flagella FWEVVSDEHGIDPTGTYHGDSDLQLER
(Pan et al. Hippler 2011)Purified Flagella FWEVVSDEHGIDPTGTYHGDSDLQLER
(Pan et al. Hippler 2011)Purified Flagella FWEVVSDEHGIDPTGTYHGDSDLQLER
(Pan et al. Hippler 2011)Purified Flagella FWEVVSDEHGIDPTGTYHGDSDLQLER
(Pan et al. Hippler 2011)Purified Flagella FWEVVSDEHGIDPTGTYHGDSDLQLER
(Pan et al. Hippler 2011)Purified Flagella LHFFMVGFTPLTSRGSQQYR
(Pan et al. Hippler 2011)Purified Flagella LHFFMVGFTPLTSRGSQQYR
(Pan et al. Hippler 2011)Purified Flagella LHFFMVGFTPLTSRGSQQYR
(Pan et al. Hippler 2011)Purified Flagella SGPYGQIFRPDNFVFGQTGAGNNWAK
(Pan et al. Hippler 2011)Purified Flagella SGPYGQIFRPDNFVFGQTGAGNNWAK
(Pan et al. Hippler 2011)Purified Flagella SGPYGQIFRPDNFVFGQTGAGNNWAK
ChlamyFP Peptides
Fraction Sequence
(click on peptide to see other models containing this peptide)
T28NSSYFVEWIPNNVK
T27NMMCAADPR
T11LAVNLIPFPR
T06ALTVPELTQQMWDAK
T01FPGQLNADLR
C33EVDEQMLNVQNK
C28VSEQFTAMFR
C26GHYTEGAELIDSVLDVVRK
C26RVSEQFTAMFR
C25MSTKEVDEQMLNVQNK
C25VSEQFTAMFRR
C25SSVCDIPPKGLK
C24MSATFIGNSTAIQEMFK
C24EIVHIQGGQCGNQIGAK
C23MSATFIGNSTAIQEMFKR
C22SGPYGQIFRPDNFVFGQTGAGNNWAK
C15YLTASALFR
C12FWEVVSDEHGIDPTGTYHGDSDLQLER
C09LHFFMVGFTPLTSR
B24KLAVNLIPFPR
B22SSVCDIPPK
B22IREEYPDR
B10INVYFNEATGGR
B10AILMDLEPGTMDSVR
A31GHYTEGAELIDSVLDVVR
A28MMLTFSVVPSPK
A27MREIVHIQGGQCGNQIGAK
A27RVSEQFTAMFRR
© Gregory J. Pazour