The Chlamydomonas Flagellar Proteome Project
ChlamyFPv5 (gene name | annotation): LF5 | "LF5 (FAP247), Flagellar Associated Protein 247; Similar to MAPK3, Mitogen-Activated Protein Kinase 3"
Aliases: LF5; FAP247; CFAP247
Structure:
References: "LF5, (Tam, Ranum, and Lefebvre, 2013); PF27? Rescues pf27 (Alford and Sale abstract #94 Chlamy 2012)"
CrFP Total Peptides = 4 (0 Axonemal Peptides | 0 M+M Peptides | 4 KCl Peptides | 0 Tergitol Peptides)
Wang Peptides (Wang et al. Pan 2017) = 8 (enriched 1.23 fold by ciliary shortening)
Phytozome (gene name | defline): LF5 | (1 of 1) PTHR24056:SF111 - CYCLIN-DEPENDENT KINASE-LIKE 5
Annotation v5.6 Symbol | Defline: LF5 | Flagellar Associated Protein 247
Annotation v5.6 Description: Flagellar Associated Protein, found in the flagellar proteome [PMID: 15998802]; SSA1; identified by comparative genomics as being present only in organisms having cilia, including non-motile cilia; may have role in sensing, structure and/or assembly (SSA)
Annotation v5.6 Synonyms: g13235.t1#FAP247
Steve King Dynein Anotation: |
Yuqing Hou's Human Homologs: CDKL5, NP_003150.1, cyclin-dependent kinase-like 5 [Homo sapiens] (Blast E value: 0 | Reprocal Best Match? Yes)
Albee et al. Dutcher 2013 Human Homolog: CDKL5, cyclin dependent kinase 5 transcript variant
Lipid Modifications (N-Myristoylation: MGXXXS/T, S-Geranylgeranylation: CAAX , S-Geranylgeranylation: CC/CXC, S-Farnesylation: CAAX predicted by GPS-Lipid):
Fold Upregulated Post Deflagellation (Albee et al. Dutcher 2013) at 3 min: 7.81; at 10 min: 14.43; at 30 min: 5.63; at 60 min: 1.83
Importance of XAP5 for expression (Li et al. Hu 2018) Wild Type: 517.72 FPKM | XAP Mutant: 85.72 FPKM | Fold Change: -2.59
CLIP Library: Cre12.g538300
Phytozome Transcript: Cre12.g538300.t1.1
PhosphoPeptides
Protein Reference Source Peptide pLOGO
(Pan et al. Hippler 2011)Purified Flagella HEPMDHDMSDNESTASTVAVAR
ChlamyFP Peptides
Fraction Sequence
(click on peptide to see other models containing this peptide)
C41AAAAAAAASGGK
C32ALLAVDPSAR
C27DIKPENLLISPSAAGGVGK
C27AASSSAAPADSGVR
© Gregory J. Pazour