The Chlamydomonas Flagellar Proteome Project
ChlamyFPv5 (gene name | annotation): RSP16 | "RSP16, Radial Spoke Protein 16"
Aliases: RSP16; HSP40; PF33
Structure: Radial Spoke
References: Radial spoke protein (Yang et al. Witman 2006) and (Zhu et al. Yang 2018); Ciliary level increases during disassembly (Wang et al. Pan 2017); Mutants have altered radial spoke structure. Suggested to play roles in transport and folding of radial spokes (Zhu et al. Yang 2018)
CrFP Total Peptides = 20 (8 Axonemal Peptides | 1 M+M Peptides | 1 KCl Peptides | 10 Tergitol Peptides)
Wang Peptides (Wang et al. Pan 2017) = 10 (enriched 3 fold by ciliary shortening)
Jordan Peptides (Jordan et al. Pigino 2018) = Wild Type M+M: 8 | Dhc1b-3 M+M: 9 | Ift139-2 M+M: 7 (Total Unique Peptides)
Picariello Peptides (Picariello et al. Witman 2019) = Wild Type M+M: 6 | Ift140 ΔN-terminal WD repeat M+M: 16 (Total Unique Peptides)
Phytozome (gene name | defline): RSP16 | Radial spoke protein 16
Annotation v5.6 Symbol | Defline: RSP16 | Radial Spoke Protein 16
Annotation v5.6 Description: A structural HSP40 (DnaJ) in the radial spoke stalk [PMID: 16507594, PMID: 15563613; GI:56404248]
Annotation v5.6 Synonyms: Cre12.g511050.t1.1#g12327.t1
Steve King Dynein Anotation: |
Yuqing Hou's Human Homologs: DNAJB13, , (Blast E value: | Reprocal Best Match? )
Albee et al. Dutcher 2013 Human Homolog: DNAJB13, dnaJ homolog subfamily B member 13
Lipid Modifications (N-Myristoylation: MGXXXS/T, S-Geranylgeranylation: CAAX , S-Geranylgeranylation: CC/CXC, S-Farnesylation: CAAX predicted by GPS-Lipid):
Fold Upregulated Post Deflagellation (Albee et al. Dutcher 2013) at 3 min: 9.34; at 10 min: 15.09; at 30 min: 15.05; at 60 min: 3.55
Importance of XAP5 for expression (Li et al. Hu 2018) Wild Type: 864.43 FPKM | XAP Mutant: 168.56 FPKM | Fold Change: -2.36
CLIP Library: Cre12.g511050
Phytozome Transcript: Cre12.g511050.t1.2
PhosphoPeptides
Protein Reference Source Peptide pLOGO
ChlamyFP Peptides
Fraction Sequence
(click on peptide to see other models containing this peptide)
T10KVLLFSGEYVEEER
T10YHPDINKDGAAGDEFLR
T10TRGLDYYEVMGLTR
T10GLDYYEVMGLTR
T10VLLFSGEYVEEERQLTVDVKPGLPTGTR
T10DYEAAFKHDLK
T10SAFFLPPPTEQNEETKK
T10SANDIDIR
T10SAFFLPPPTEQNEETK
T10KEPGPVVFVLKPKPHPR
C29VLLFSGEYVEEER
B26FVFEGEGNKTPK
A30VTLPLHHALIGTTLDIR
A30GFYDLYGEDALK
A30QLTVDVKPGLPTGTR
A30LALKYHPDINKDGAAGDEFLR
A30DYEAAFK
A30FNPEESPK
A30ALRDYEAAFK
A30GFYDLYGEDALKDGISDGNGGLK
© Gregory J. Pazour