The Chlamydomonas Flagellar Proteome Project
ChlamyFPv5 (gene name | annotation): FAP221 | "FAP221, Coiled-Coil Flagellar Associated Protein 221; HC4 of C1d projection on central pair; very Similar to FAP360 - not clear if this is a recent gene duplicatoin or an assembly artifact; Similar to mammalian primary ciliary dyskinesia protein 1 (Pcdp1)"
Aliases: FAP221; Pcdp1; CFAP221
Structure: Central Pair
References: "Pcdp1: HC4 of C1d projection on central pair (DiPetrillo and Smith 2010)"
CrFP Total Peptides = 13 (12 Axonemal Peptides | 0 M+M Peptides | 0 KCl Peptides | 1 Tergitol Peptides)
Wang Peptides (Wang et al. Pan 2017) = 11 (enriched 1.14 fold by ciliary shortening)
Phytozome (gene name | defline): FAP221 | (1 of 2) PTHR12949 - RNA POLYMERASE III DNA DIRECTED -RELATED
O. Vallon Gene Name | Defline: FAP221 | Coiled-Coil Flagellar Associated Protein 221
O. Vallon Description: HC4 of C1d projection on central pair; Similar to mammalian primary ciliary dyskinesia protein 1 (Pcdp1); this gene model is probably a fusion between RNA polymerase III subunit RPC82 and a protein very similar to FAP221 Cre49.g761347, probably due to an
O. Vallon Synonyms: g11833.t1
Steve King Dynein Anotation: |
Yuqing Hou's Human Homologs: CFAP221, NP_001257978.1, primary ciliary dyskinesia protein 1 [Homo sapiens] (Blast E value: 0 | Reprocal Best Match? Yes)
Albee et al. Dutcher 2013 Human Homolog: POLR3C, POLR3C protein
Lipid Modifications (N-Myristoylation: MGXXXS/T, S-Geranylgeranylation: CAAX , S-Geranylgeranylation: CC/CXC, S-Farnesylation: CAAX predicted by GPS-Lipid):
Fold Upregulated Post Deflagellation (Albee et al. Dutcher 2013) at 3 min: 2.15; at 10 min: 6.64; at 30 min: 8.7; at 60 min: 2.48
Importance of XAP5 for expression (Li et al. Hu 2018) Wild Type: FPKM | XAP Mutant: FPKM | Fold Change:
CLIP Library: Cre11.g476376
Phytozome Transcript: Cre11.g476376.t1.1
PhosphoPeptides
Protein Reference Source Peptide pLOGO
Cre11.g476376.t1.1--T684 (Werth et al. Hicks 2018)Wild Type Whole Cell ExtractLAEEAANPVLLVSESDRPGTAPTKLAEEAANPVLLVSESDRPGpTAPTK
ChlamyFP Peptides
Fraction Sequence
(click on peptide to see other models containing this peptide)
T30VLGGSAGGGGGGR
A14LAEEAANPVLLVSESDRPGTAPTK
A14HEAVASGGVR
A14HGGGGGATASGLK
A14LTGSAFPGAGR
A14GGPGLSDSWLVR
A13KAAAAEQQAALEK
A13AAAAEQQAALEK
A13LQLALAEEAAR
A13TAAAATANGKHGGGGGATASGLK
A13QYDVYDSAR
A13GLPVNPGGGTLR
A13GDPSASPSSPSTSSAAPTAYR