The Chlamydomonas Flagellar Proteome Project
ChlamyFPv5 (gene name | annotation): FAP18 | "FAP18, Flagellar Associated Protein 18"
Aliases: FAP18
Structure:
References: Ciliary level increases during disassembly (Wang et al. Pan 2017)"
CrFP Total Peptides = 16 (1 Axonemal Peptides | 6 M+M Peptides | 6 KCl Peptides | 3 Tergitol Peptides)
Wang Peptides (Wang et al. Pan 2017) = 10 (enriched 1.54 fold by ciliary shortening)
Phytozome (gene name | defline): FAP18 | "Flagellar Associated Protein, serine endopeptidase"
O. Vallon Gene Name | Defline: FAP18 | Flagellar Associated Protein 18
O. Vallon Description: Flagellar Associated Protein, found in the flagellar proteome; high E/A/P content. Similar to IgA-specific serine peptidase
O. Vallon Synonyms: Cre10.g450450.t1.1#g11070.t1
Steve King Dynein Anotation: |
Yuqing Hou's Human Homologs: none, , (Blast E value: | Reprocal Best Match? )
Albee et al. Dutcher 2013 Human Homolog: ,
Lipid Modifications (N-Myristoylation: MGXXXS/T, S-Geranylgeranylation: CAAX , S-Geranylgeranylation: CC/CXC, S-Farnesylation: CAAX predicted by GPS-Lipid):
Fold Upregulated Post Deflagellation (Albee et al. Dutcher 2013) at 3 min: 4.63; at 10 min: 9.08; at 30 min: 10.05; at 60 min: 3.46
Importance of XAP5 for expression (Li et al. Hu 2018) Wild Type: 263.21 FPKM | XAP Mutant: 61.8 FPKM | Fold Change: -2.09
CLIP Library: Cre10.g450450
Phytozome Transcript: Cre10.g450450.t1.2
PhosphoPeptides
Protein Reference Source Peptide pLOGO
Cre10.g450450.t1.2--S327 (Werth et al. Hicks 2018)Wild Type Whole Cell ExtractLKPSPSQDERLKPpSPSQDER
Cre10.g450450.t1.2--S329 (Werth et al. Hicks 2018)Wild Type Whole Cell ExtractLKPSPSQDERLKPSPpSQDER
Cre10.g450450.t1.2--S371 (Werth et al. Hicks 2018)Wild Type Whole Cell ExtractEQQAPNESLAPMSVHPPSPSKEQQAPNESLAPMSVHPPpSPSK
Cre10.g450450.t1.2--S327(Werth et al. Hicks 2018) AZD-insensitive Whole Cell ExtractLKPSPSQDER
FAP18, High E/A/P Content Protein(Pazour unpublished)Purified FlagellaSAATPPTKKSAApTPPTKK
ChlamyFP Peptides
Fraction Sequence
(click on peptide to see other models containing this peptide)
T15KISQDEYDAMITR
T11SRQNSMVGEQPK
T06SAKSEAVGSTSVK
C30QNSMVGEQPK
C19GYKAGEEGSTSVK
C19SIPPEAATVFNTK
C18MTNAYVDKMTQEQGGK
C18SNAAGEHPDVVTDINDSPEAHAEVDEMAQAQAR
C18QGPWLSGTYKLPK
B34SEAVGSTSVK
B28YTEEGKETWQPVK
B28SSGTNASVR
B28TQEELAAK
B25AGEEGSTSVK
B15SVTDSDRIQLMTLK
A39ISQDEYDAMITR