The Chlamydomonas Flagellar Proteome Project
ChlamyFPv5 (gene name | annotation): FAP12 | "FAP12, Lipase Domain Flagellar Associated Protein 12; triacylglycerol lipase"
Aliases: FAP12
Structure:
References:
CrFP Total Peptides = 20 (0 Axonemal Peptides | 7 M+M Peptides | 4 KCl Peptides | 9 Tergitol Peptides)
Wang Peptides (Wang et al. Pan 2017) = 20 (enriched 1.37 fold by ciliary shortening)
Phytozome (gene name | defline): FAP12 | Triacylglycerol lipase and Flagellar Associated Protein
O. Vallon Gene Name | Defline: FAP12 | Lipase Domain Flagellar Associated Protein 12
O. Vallon Description: triacylglycerol lipase
O. Vallon Synonyms: Cre02.g126050.t1.1#g9673.t1#Cre02.g126050.t1.2
Steve King Dynein Anotation: |
Yuqing Hou's Human Homologs: DAGLB, NP_001136408.1, Phylogenetic analysis would be necessary to distinguish between alpha and beta isoforms; sn1-specific diacylglycerol lipase beta isoform 2 [Homo sapiens] (Blast E value: 2.00E-13 | Reprocal Best Match? Yes)
Albee et al. Dutcher 2013 Human Homolog: DAGLB, KCCR13L, isoform CRA_a
Lipid Modifications (N-Myristoylation: MGXXXS/T, S-Geranylgeranylation: CAAX , S-Geranylgeranylation: CC/CXC, S-Farnesylation: CAAX predicted by GPS-Lipid): N-Myristoylation: MGXXXS/T
Fold Upregulated Post Deflagellation (Albee et al. Dutcher 2013) at 3 min: 10.95; at 10 min: 18.28; at 30 min: 15.78; at 60 min: 4.05
Importance of XAP5 for expression (Li et al. Hu 2018) Wild Type: 7078.96 FPKM | XAP Mutant: 719.28 FPKM | Fold Change: -3.3
CLIP Library: Cre09.g390615
Phytozome Transcript: Cre09.g390615.t1.1
PhosphoPeptides
Protein Reference Source Peptide pLOGO
(Pan et al. Hippler 2011)Purified Flagella AASDPGGLLKDLLDEK
(Pan et al. Hippler 2011)Purified Flagella AASKLGQPAVAVVADR
(Pan et al. Hippler 2011)Purified Flagella AASKLGQPAVAVVADRER
(Pan et al. Hippler 2011)Purified Flagella KAASDPGGLLKDLLDEK
(Pan et al. Hippler 2011)Purified Flagella QVELQSLQDTGYHVDAK
ChlamyFP Peptides
Fraction Sequence
(click on peptide to see other models containing this peptide)
T21AALVVSMAAEPTPK
T02EWEGGYAHESVSLGAR
T01MDKAEDAVDAAAASNEKR
T01GTANMKDVLTDLAGAAR
T01VFDEIKEYVLNLK
T01ELVEEAHPYCTTIVHDADLVPR
T01LGQPAVAVVADRER
T01AQNPSFAVR
T01LMFLSKPEKGNGPYTISK
C24KEAAEGESFGSDLK
C24SLPDHLAATEDPTNQVEK
C23KVFDEIKEYVLNLK
C23WADAVYDKEEVEDPKAALVVSMAAEPTPK
B22DGGDGNTASAPQPK
B22KEAAEGESFGSDLKSFLIK
B22RLYAPGR
B21TLKVEDVAYFR
B20AEDAVDAAAASNEK
B19GSMASETSK
B18QVELQSLQDTGYHVDAK