The Chlamydomonas Flagellar Proteome Project
ChlamyFPv5 (gene name | annotation): ADF3 | "ADF3 (FAP16), necessary for axonemal severing; Similar to Echinoderm Microtubule Binding Protein"
Aliases: ADF3; FAP16
Structure:
References: "ADF3, necessary for axonemal severing (Hilton et al. Quarmby 2016)"
CrFP Total Peptides = 11 (0 Axonemal Peptides | 11 M+M Peptides | 0 KCl Peptides | 0 Tergitol Peptides)
Wang Peptides (Wang et al. Pan 2017) = 11 (enriched 1.23 fold by ciliary shortening)
Phytozome (gene name | defline): FAP16 | Flagellar Associated Protein
Annotation v5.6 Symbol | Defline: ADF3 | necessary for axonemal severing
Annotation v5.6 Description: ADF3, necessary for axonemal severing (PubMed ID:27520959 Hilton et al. Quarmby 2016); Similar to Echinoderm Microtubule Binding Protein
Annotation v5.6 Synonyms: g7025.t1
Steve King Dynein Anotation: |
Yuqing Hou's Human Homologs: EML5, NP_899243, echinoderm microtubule-associated protein-like 3 [Homo sapiens]or echinoderm microtubule-associated protein-like 5 [Homo sapiens] (Blast E value: 0.00 | Reprocal Best Match? Yes)
Albee et al. Dutcher 2013 Human Homolog: EML3, echinoderm microtubule associated protein like 3, isoform CRA_a
Lipid Modifications (N-Myristoylation: MGXXXS/T, S-Geranylgeranylation: CAAX , S-Geranylgeranylation: CC/CXC, S-Farnesylation: CAAX predicted by GPS-Lipid):
Fold Upregulated Post Deflagellation (Albee et al. Dutcher 2013) at 3 min: 4.61; at 10 min: 18.27; at 30 min: 16.53; at 60 min: 1.96
Importance of XAP5 for expression (Li et al. Hu 2018) Wild Type: FPKM | XAP Mutant: FPKM | Fold Change:
CLIP Library: Cre06.g303400
Phytozome Transcript: Cre06.g303400.t1.1
PhosphoPeptides
Protein Reference Source Peptide pLOGO
Cre06.g303400.t1.1--S1575 (Werth et al. Hicks 2018)Wild Type Whole Cell ExtractDQLTEEQLQEAGASPTKRDQLTEEQLQEAGApSPTKR
Cre06.g303400.t1.1--T1577(Werth et al. Hicks 2018) AZD-insensitive Whole Cell ExtractDQLTEEQLQEAGASPTKR
ChlamyFP Peptides
Fraction Sequence
(click on peptide to see other models containing this peptide)
B07LATTELEYMMR
B07RDQLTEEQLQEAGASPTKR
B07MFNYPVVADDAPFK
B07TEENAGWTPGGKVQDDLLR
B07LPDLNVWTKIPGTNQYASIR
B07QYIPELK
B06KAEEPPIVVALDAHEALNR
B06TEGGGGASTSGAALGGVGGR
B06TGGMAAALGVGQTQGR
B06IPGTNQYASIR
B06GGSAGGATTPVSSSPAPASNGKPVSVAGGSK
© Gregory J. Pazour