The Chlamydomonas Flagellar Proteome Project
ChlamyFPv5 (gene name | annotation): EEF1A3 | "Elongation Factor 1 alpha"
Aliases: EEF1A3
Structure: Transition Zone
References: Ciliary level increases during disassembly (Wang et al. Pan 2017); Transition Zone Protein (19 spectral counts [Diener et al. Rosenbaum 2015])
CrFP Total Peptides = 26 (0 Axonemal Peptides | 16 M+M Peptides | 8 KCl Peptides | 2 Tergitol Peptides)
Wang Peptides (Wang et al. Pan 2017) = 15 (enriched 2 fold by ciliary shortening)
Jordan Peptides (Jordan et al. Pigino 2018) = Wild Type M+M: 34 | Dhc1b-3 M+M: 34 | Ift139-2 M+M: 23 (Total Unique Peptides)
Picariello Peptides (Picariello et al. Witman 2019) = Wild Type M+M: 42 | Ift140 ΔN-terminal WD repeat M+M: 38 (Total Unique Peptides)
Phytozome (gene name | defline): | (1 of 3) K03231 - elongation factor 1-alpha (EEF1A)
Annotation v5.6 Symbol | Defline: EEF1A3 | Putative eukaryotic translation initiation factor 1 alpha
Annotation v5.6 Description: EEF1a; high similarity to EF1A2; found in the flagellar proteome [PMID: 1599882]
Annotation v5.6 Synonyms: Cre06.g263450.t1.1#g5919.t2#EEF1A3
Steve King Dynein Anotation: (no data) | (no data)
Yuqing Hou's Human Homologs: multiple, (no data), (no data) (Blast E value: (no data) | Reprocal Best Match? (no data))
Albee et al. Dutcher 2013 Human Homolog: (no data), (no data)
Lipid Modifications (N-Myristoylation: MGXXXS/T, S-Geranylgeranylation: CAAX , S-Geranylgeranylation: CC/CXC, S-Farnesylation: CAAX predicted by GPS-Lipid): (no data)
Fold Upregulated Post Deflagellation (Albee et al. Dutcher 2013) at 3 min: (no data); at 10 min: (no data); at 30 min: (no data); at 60 min: (no data)
Importance of XAP5 for expression (Li et al. Hu 2018) Wild Type: 7986.13 FPKM | XAP Mutant: 2447.88 FPKM | Fold Change: -1.71
CLIP Library: Cre06.g263450
Phytozome Transcript: Cre06.g263450.t2.1
PhosphoPeptides
Protein Reference Source Peptide pLOGO
Cre06.g263450.t2.1--S290 (Werth et al. Hicks 2018)Wild Type Whole Cell ExtractTGDVMILKSDQTLKTGDVMILKpSDQTLK
ChlamyFP Peptides
Fraction Sequence
(click on peptide to see other models containing this peptide)
T22IAFLDGNTAVMLGK
T22LINLLGVK
C26IQVHTLLDALNSFVVVPERK
C26STNMTWYSGQEVVNLKGEK
C26RVDKAGPGDNVGMNIK
C26GLDKGNMPR
C26GVTIACTTK
C26TDAPLRLPISGAYK
C26ERYDEIANEMR
C26MDSDTAGYKK
B41SVPILPISGWLGDNLITK
B31AGEIQGQTR
B23QLIVGVNKMDSDTAGYK
B23AGPGDNVGMNIK
B23STNMTWYSGQEVVNLK
B23IVKDFTAQIQTLDIPGEVKK
B23DFTAQIQTLDIPGEVK
B23TGDVMILK
B23DFTAQIQTLDIPGEVKK
B23KGYSPIGFVR
B23YDEIANEMR
B23MDSDTAGYK
B23VDKAGPGDNVGMNIK
B23VGWKDDFVNK
B23IVKDFTAQIQTLDIPGEVK
B23GYSPIGFVR
© Gregory J. Pazour