The Chlamydomonas Flagellar Proteome Project
ChlamyFPv5 (gene name | annotation): TUA2 | "TUA2, alpha-2 Tubulin"
Aliases: TUA2
Structure: Axoneme; Transition Zone
References: (Silflow et al. Ranum 1985); Transition Zone Protein (41 spectral counts [Diener et al. Rosenbaum 2015]); Ubiquitinated (Wang et al. Huang 2019)
CrFP Total Peptides = 32 (15 Axonemal Peptides | 4 M+M Peptides | 10 KCl Peptides | 3 Tergitol Peptides)
Wang Peptides (Wang et al. Pan 2017) = (no data) (enriched (no data) fold by ciliary shortening)
Jordan Peptides (Jordan et al. Pigino 2018) = Wild Type M+M: 23 | Dhc1b-3 M+M: 25 | Ift139-2 M+M: 24 (Total Unique Peptides)
Picariello Peptides (Picariello et al. Witman 2019) = Wild Type M+M: 25 | Ift140 ΔN-terminal WD repeat M+M: 28 (Total Unique Peptides)
Phytozome (gene name | defline): TUA2 | Alpha tubulin 2
Annotation v5.6 Symbol | Defline: TUA2 | Alpha tubulin 2
Annotation v5.6 Description: GI:27358004; PMID: 3855249, PMID: 3460413, PMID: 14676280
Annotation v5.6 Synonyms: g4512.t1#Cre04.g216850.t1.1
Steve King Dynein Anotation: (no data) | (no data)
Yuqing Hou's Human Homologs: TUBA1C, (no data), (no data) (Blast E value: (no data) | Reprocal Best Match? (no data))
Albee et al. Dutcher 2013 Human Homolog: TUBA1C, tubulin alpha-1C chain
Lipid Modifications (N-Myristoylation: MGXXXS/T, S-Geranylgeranylation: CAAX , S-Geranylgeranylation: CC/CXC, S-Farnesylation: CAAX predicted by GPS-Lipid): (no data)
Fold Upregulated Post Deflagellation (Albee et al. Dutcher 2013) at 3 min: 4.86; at 10 min: 6.58; at 30 min: 7.77; at 60 min: 6.36
Importance of XAP5 for expression (Li et al. Hu 2018) Wild Type: 3970.74 FPKM | XAP Mutant: 946.81 FPKM | Fold Change: -2.07
CLIP Library: Cre04.g216850
Phytozome Transcript: Cre04.g216850.t1.2
PhosphoPeptides
Protein Reference Source Peptide pLOGO
(Pan et al. Hippler 2011)Purified Flagella AYHEQLSVAEITNAAFEPASMMVK
(Pan et al. Hippler 2011)Purified Flagella AYHEQLSVAEITNAAFEPASMMVK
(Pan et al. Hippler 2011)Purified Flagella QLFHPEQLISGKEDAANNFAR
(Pan et al. Hippler 2011)Purified Flagella SLDIERPTYTNLNR
ChlamyFP Peptides
Fraction Sequence
(click on peptide to see other models containing this peptide)
T07AFVHWYVGEGMEEGEFSEAR
T07RAFVHWYVGEGMEEGEFSEAR
T01AFVHWYVGEGMEEGEFSEAREDLAALEK
C31DVNASVATIK
C27AVCMISNSTAIGEIFSR
C27QLFHPEQLISGKEDAANNFAR
C27EIVDLALDR
C26KLADNCTGLQGFLVFNAVGGGTGSGLGSLLLER
C26DVNASVATIKTK
C25IHFMLSSYAPIISAEK
C25RTIQFVDWCPTGFK
C25LDHKFDLMYAK
C24AYHEQLSVAEITNAAFEPASMMVK
B37CGINYQPPTVVPGGDLAK
B37FDLMYAK
B22TIQFVDWCPTGFK
B22LSVDYGKK
A33CIFLDLEPTVVDEVR
A33SLDIERPTYTNLNR
A33QLFHPEQLISGK
A32EDAANNFAR
A27GHYTIGKEIVDLALDR
A27LADNCTGLQGFLVFNAVGGGTGSGLGSLLLER
A27EDLAALEKDFEEVGAESAEGAGEGEGEEY
A27LDHKFDLMYAKR
A27GDVVPKDVNASVATIK
A27FDLMYAKR
A26EDLAALEK
A25EIVDLALDRIR
A14LIAQVISSLTASLR
A02TIGGGDDAFNTFFSETGAGK
A02GHYTIGKEIVDLALDRIR
© Gregory J. Pazour