The Chlamydomonas Flagellar Proteome Project
ChlamyFPv5 (gene name | annotation): FAP251 | "FAP251, Flagellar Associated Protein 251; CaM-IP4; Associated with the CaM- and spoke-Associated CSC complex at the base of the radial spoke "
Aliases: FAP251; CaM-IP4; CFAP251; WDR66
Structure: Central Pair
References: "CaM-IP4, associated with the CaM- and spoke-associated CSC complex at the base of the radial spoke (Dymek and Smith 2007); basal structure of RS3 (Urbanska et al. Wloga 2015); mutations lead to male infertility in humans (Auguste et al. Mitchell 2018)(Li et al. Cao 2018)"
CrFP Total Peptides = 17 (13 Axonemal Peptides | 0 M+M Peptides | 0 KCl Peptides | 4 Tergitol Peptides)
Wang Peptides (Wang et al. Pan 2017) = 12 (enriched 1 fold by ciliary shortening)
Jordan Peptides (Jordan et al. Pigino 2018) = Wild Type M+M: 6 | Dhc1b-3 M+M: 14 | Ift139-2 M+M: 6 (Total Unique Peptides)
Phytozome (gene name | defline): FAP251 | Flagellar Associated Protein
Annotation v5.6 Symbol | Defline: FAP251 | Flagellar Associated Protein
Annotation v5.6 Description: Flagellar Associated Protein; found in the flagellar proteome; identified in the flagellar basal body proteome [PMID: 15137946]; upregulated by deflagellation; associated with the CaM- and spoke-Associated CSC complex at the base of the radial spoke
Annotation v5.6 Synonyms: Cre03.g185650.t1.1#g3847.t1
Steve King Dynein Anotation: |
Yuqing Hou's Human Homologs: WDR66, NP_001171474.1, WD repeat-containing protein 66 isoform 2 [Homo sapiens]; CaM-IP4 by Beth Smith (Chlamy) (Blast E value: 0 | Reprocal Best Match? Yes)
Albee et al. Dutcher 2013 Human Homolog: WDR66, WD repeat-containing protein 66 isoform 1
Lipid Modifications (N-Myristoylation: MGXXXS/T, S-Geranylgeranylation: CAAX , S-Geranylgeranylation: CC/CXC, S-Farnesylation: CAAX predicted by GPS-Lipid):
Fold Upregulated Post Deflagellation (Albee et al. Dutcher 2013) at 3 min: 6.09; at 10 min: 10.71; at 30 min: 11.85; at 60 min: 2.95
Importance of XAP5 for expression (Li et al. Hu 2018) Wild Type: 329.5 FPKM | XAP Mutant: 88.05 FPKM | Fold Change: -1.9
CLIP Library: Cre03.g185650
Phytozome Transcript: Cre03.g185650.t1.2
PhosphoPeptides
Protein Reference Source Peptide pLOGO
ChlamyFP Peptides
Fraction Sequence
(click on peptide to see other models containing this peptide)
T32VYFWSWAPGSPR
T32DVPGTVPVDLVPDLMR
T32FMVPDFVVATR
T32SIAISYDGR
A16SHVVTADTGPEALLVVWNVR
A16SPSAASDGAFLAYR
A16SATAAEGAGGEAR
A16TMGLIAHPGEVR
A16WAAVLGDPDLLR
A16GSSVLDSLLADVVDLAAHPTR
A16LAHADAADTLNR
A16SHHGPIASVVFGESPSGQTR
A16LLSVGGDGR
A16DYFVYAQIK
A16IVSVQSASFEEYDADRR
A16FQYYSPPLR
A16TQGEDALEPR
© Gregory J. Pazour