The Chlamydomonas Flagellar Proteome Project
ChlamyFPv5 (gene name | annotation): PACRG | "Parkin Co-Regulated Gene Protein"
Aliases: PACRG
Structure: Axoneme
•Axonemal localization of Chlamydomonas PACRG, a homologue of the human Parkin-coregulated gene product (Ikeda et al. Kamiya 2007)
•Microtubule binding protein PACRG plays a role in regulating specific ciliary dyneins during microtubule sliding (Mizuno et al. Smith 2016)
•Transition Zone Protein (2 spectral counts [Diener et al. Rosenbaum 2015])
•PACRG and FAP20 form the inner junction of axonemal doublet microtubules and regulate ciliary motility (Dymek et al. Smith 2019)
•Deletion of the Parkin coregulated gene causes male sterility in the quaking(viable)mouse mutant (Lorenzetti et al. Justice 2004)
•The Parkin co-regulated gene product, PACRG, is an evolutionarily conserved axonemal protein that functions in outer-doublet microtubule morphogenesis (Dawe et al. Gull 2005)
•Deletion of the Parkin co-regulated gene causes defects in ependymal ciliary motility and hydrocephalus in the quakingviable mutant mouse (Wilson et al. Lockhart 2010)
•Generation and characterisation of a parkin-Pacrg knockout mouse line and a Pacrg knockout mouse line (Stephenson et al. Lockhart 2018)
•Outer doublet microtubule inner luminal protein (MIP) associated with the inner junction. Repeats with 8 nm periodicity (Ma et al. Zhang 2019)
•The inner junction complex of the cilia is an interaction hub that involves tubulin post-translational modifications (Khalifa et al. Bui 2020)
•Subunit of the axonemal IJ protofilament (IJ PF) (Khalifa et al. Bui 2020)
CrFP Total Peptides = 20 (9 Axonemal Peptides | 0 M+M Peptides | 2 KCl Peptides | 9 Tergitol Peptides)
Wang Peptides (Wang et al. Pan 2017) = 8 (enriched 1 fold by ciliary shortening)
Jordan Peptides (Jordan et al. Pigino 2018) = Wild Type M+M: 3 | Dhc1b-3 M+M: 3 | Ift139-2 M+M: 3 (Total Unique Peptides)
Picariello Peptides (Picariello et al. Witman 2019) = Wild Type M+M: 9 | Ift140 ΔN-terminal WD repeat M+M: 14 (Total Unique Peptides)
Zhao Central Apparatus Project (Zhao et al. Witman 2019) = Wild Type Axo: 124 | Pf18 Axo: 132 (Total Unique Peptides, summed from two biological replicates for each. Proteins found only in this dataset needed 10 or more peptides to be included)
Phytozome (gene name | defline): | 1
Annotation v5.6 Symbol | Defline: | Parkin Co-Regulated Gene Protein
Annotation v5.6 Description: NULL
Annotation v5.6 Synonyms: Cre02.g113400.t1.1#g2336.t1#BUG21
Steve King Dynein Anotation: (no data) | (no data)
Yuqing Hou's Human Homologs: PACRG, (no data), (no data) (Blast E value: (no data) | Reprocal Best Match? (no data))
Albee et al. Dutcher 2013 Human Homolog: PACRG, parkin coregulated gene protein isoform 2
Lipid Modifications (N-Myristoylation: MGXXXS/T, S-Geranylgeranylation: CAAX , S-Geranylgeranylation: CC/CXC, S-Farnesylation: CAAX predicted by GPS-Lipid): (no data)
Fold Upregulated Post Deflagellation (Albee et al. Dutcher 2013) at 3 min: 8.62; at 10 min: 13.1; at 30 min: 10.51; at 60 min: 3.1
Importance of XAP5 for expression (Li et al. Hu 2018) Wild Type: 4764.75 FPKM | XAP Mutant: 1459.61 FPKM | Fold Change: -1.71
CLIP Library: Cre02.g113400
Phytozome Transcript: Cre02.g113400.t1.2
Protein Reference Source Peptide pLOGO
Cre02.g113400.t1.2--S103 (Werth et al. Hicks 2018)Wild Type Whole Cell ExtractKPSPPQAGAYKKPpSPPQAGAYK
Cre02.g113400.t1.2--S42 (Werth et al. Hicks 2018)Wild Type Whole Cell ExtractAPPAANLSGTGSCFDTTSLSPARAPPAANLSGTGSCFDTTSLpSPAR
Cre02.g113400.t1.2--S42(Werth et al. Hicks 2018) AZD-insensitive Whole Cell ExtractAPPAANLSGTGSCFDTTSLSPAR
Cre02.g113400.t1.2--S103(Werth et al. Hicks 2018) AZD-insensitive Whole Cell ExtractKPSPPQAGAYK
Parkin Co-Regulated Gene Protein(Pazour unpublished)Purified FlagellaKPSPPQAGAYKKPpSPPQAGAYK
Parkin Co-Regulated Gene Protein(Pazour unpublished)Purified FlagellaKPSPPQAGAYKRKPpSPPQAGAYKR
Cre02.g113400.t1.2--S103 (Ford et al. Hicks 2020)Whole Cell Extract
Cre02.g113400.t1.2--S42 (Ford et al. Hicks 2020)Whole Cell Extract
ChlamyFP Peptides
Fraction Sequence
(click on peptide to see other models containing this peptide)
© Gregory J. Pazour