The Chlamydomonas Flagellar Proteome Project
ChlamyFPv5 (gene name | annotation): PACRG | "Parkin Co-Regulated Gene Protein"
Aliases: PACRG
Structure: (no data)
References: "Axonemal localization of Chlamydomonas PACRG, a homologue of the human Parkin-coregulated gene product (Ikeda et al. Kamiya 2007); Microtubule binding protein PACRG plays a role in regulating specific ciliary dyneins during microtubule sliding (Mizuno et al. Smith 2016); Transition Zone Protein (2 spectral counts [Diener et al. Rosenbaum 2015]); PACRG and FAP20 form the inner junction of axonemal doublet microtubules and regulate ciliary motility (Dymek et al. Smith 2019); Deletion of the Parkin coregulated gene causes male sterility in the quaking(viable) mouse mutant (Lorenzetti et al. Justice 2004); The Parkin co-regulated gene product, PACRG, is an evolutionarily conserved axonemal protein that functions in outer-doublet microtubule morphogenesis (Dawe et al. Gull 2005); Deletion of the Parkin co-regulated gene causes defects in ependymal ciliary motility and hydrocephalus in the quakingviable mutant mouse (Wilson et al. Lockhart 2010); Generation and characterisation of a parkin-Pacrg knockout mouse line and a Pacrg knockout mouse line (Stephenson et al. Lockhart 2018); Outer doublet microtubule inner luminal protein (MIP) associated with the inner junction; Repeats with 8 nm periodicity (Ma et al. Zhang 2019)."
CrFP Total Peptides = 20 (9 Axonemal Peptides | 0 M+M Peptides | 2 KCl Peptides | 9 Tergitol Peptides)
Wang Peptides (Wang et al. Pan 2017) = 8 (enriched 1 fold by ciliary shortening)
Jordan Peptides (Jordan et al. Pigino 2018) = Wild Type M+M: 3 | Dhc1b-3 M+M: 3 | Ift139-2 M+M: 3 (Total Unique Peptides)
Picariello Peptides (Picariello et al. Witman 2019) = Wild Type M+M: 9 | Ift140 ΔN-terminal WD repeat M+M: 14 (Total Unique Peptides)
Zhao Central Apparatus Project (Zhao et al. Witman 2019) = Wild Type Axo: 124 | Pf18 Axo: 132 (Total Unique Peptides, summed from two biological replicates for each. Proteins found only in this dataset needed 10 or more peptides to be included)
Phytozome (gene name | defline): | 1
Annotation v5.6 Symbol | Defline: | Parkin Co-Regulated Gene Protein
Annotation v5.6 Description: NULL
Annotation v5.6 Synonyms: Cre02.g113400.t1.1#g2336.t1#BUG21
Steve King Dynein Anotation: (no data) | (no data)
Yuqing Hou's Human Homologs: PACRG, (no data), (no data) (Blast E value: (no data) | Reprocal Best Match? (no data))
Albee et al. Dutcher 2013 Human Homolog: PACRG, parkin coregulated gene protein isoform 2
Lipid Modifications (N-Myristoylation: MGXXXS/T, S-Geranylgeranylation: CAAX , S-Geranylgeranylation: CC/CXC, S-Farnesylation: CAAX predicted by GPS-Lipid): (no data)
Fold Upregulated Post Deflagellation (Albee et al. Dutcher 2013) at 3 min: 8.62; at 10 min: 13.1; at 30 min: 10.51; at 60 min: 3.1
Importance of XAP5 for expression (Li et al. Hu 2018) Wild Type: 4764.75 FPKM | XAP Mutant: 1459.61 FPKM | Fold Change: -1.71
CLIP Library: Cre02.g113400
Phytozome Transcript: Cre02.g113400.t1.2
Protein Reference Source Peptide pLOGO
Cre02.g113400.t1.2--S103 (Werth et al. Hicks 2018)Wild Type Whole Cell ExtractKPSPPQAGAYKKPpSPPQAGAYK
Cre02.g113400.t1.2--S42 (Werth et al. Hicks 2018)Wild Type Whole Cell ExtractAPPAANLSGTGSCFDTTSLSPARAPPAANLSGTGSCFDTTSLpSPAR
Cre02.g113400.t1.2--S42(Werth et al. Hicks 2018) AZD-insensitive Whole Cell ExtractAPPAANLSGTGSCFDTTSLSPAR
Cre02.g113400.t1.2--S103(Werth et al. Hicks 2018) AZD-insensitive Whole Cell ExtractKPSPPQAGAYK
Parkin Co-Regulated Gene Protein(Pazour unpublished)Purified FlagellaKPSPPQAGAYKKPpSPPQAGAYK
Parkin Co-Regulated Gene Protein(Pazour unpublished)Purified FlagellaKPSPPQAGAYKRKPpSPPQAGAYKR
ChlamyFP Peptides
Fraction Sequence
(click on peptide to see other models containing this peptide)
© Gregory J. Pazour