The Chlamydomonas Flagellar Proteome Project
ChlamyFPv5 (gene name | annotation): FAP19 | "FAP19, Flagellar Associated Protein 19; Similar to cGMP-dependent protein kinases"
Aliases: FAP19; CGK2; CrPKG
Structure:
References: (Wang et al. Snell 2006); Cell Cycle Gene (Breker Lieberman and Cross 2018)
CrFP Total Peptides = 10 (0 Axonemal Peptides | 10 M+M Peptides | 0 KCl Peptides | 0 Tergitol Peptides)
Wang Peptides (Wang et al. Pan 2017) = 16 (enriched 1.44 fold by ciliary shortening)
Phytozome (gene name | defline): | cGMP-dependent protein kinase
Annotation v5.6 Symbol | Defline: FAP19 | Flagellar Associated Protein 19
Annotation v5.6 Description: Similar to cGMP-dependent protein kinase; involved in signal transduction initiated by flagellar agglutination during mating [PMID: 16678098]; found in the flagellar proteome [PMID: 15998802]; ts-lethal mutant has fragile cell membrane when poked with a n
Annotation v5.6 Synonyms: CGK2#g1384.t1
Steve King Dynein Anotation: |
Yuqing Hou's Human Homologs: PRKACB, NP_002722.1, cAMP-dependent protein kinase catalytic subunit beta isoform 2 [Homo sapiens] (Blast E value: 0 | Reprocal Best Match? )
Albee et al. Dutcher 2013 Human Homolog: PRKACB, cAMP-dependent protein kinase catalytic subunit beta isoform
Lipid Modifications (N-Myristoylation: MGXXXS/T, S-Geranylgeranylation: CAAX , S-Geranylgeranylation: CC/CXC, S-Farnesylation: CAAX predicted by GPS-Lipid): N-Myristoylation: MGXXXS/T
Fold Upregulated Post Deflagellation (Albee et al. Dutcher 2013) at 3 min: 1.38; at 10 min: 2.57; at 30 min: 3.56; at 60 min: 2.22
Importance of XAP5 for expression (Li et al. Hu 2018) Wild Type: FPKM | XAP Mutant: FPKM | Fold Change:
CLIP Library: Cre02.g076900
Phytozome Transcript: Cre02.g076900.t1.1
PhosphoPeptides
Protein Reference Source Peptide pLOGO
Cre02.g076900.t1.1--S71 (Werth et al. Hicks 2018)Wild Type Whole Cell ExtractGQPGDGSGPLQQPSPAMPGAKGQPGDGpSGPLQQPSPAMPGAK
Cre02.g076900.t1.1--T857 (Werth et al. Hicks 2018)Wild Type Whole Cell ExtractYIGGDKTYTICGTPDYQAPEVIMRYIGGDKpTYTICGTPDYQAPEVIMR
Cre02.g076900.t1.1--T859 (Werth et al. Hicks 2018)Wild Type Whole Cell ExtractTYTICGTPDYQAPEVIMRTYpTICGTPDYQAPEVIMR
Cre02.g076900.t1.1--T863 (Werth et al. Hicks 2018)Wild Type Whole Cell ExtractYIGGDKTYTICGTPDYQAPEVIMRYIGGDKTYTICGpTPDYQAPEVIMR
Cre02.g076900.t1.1--S126-S128 (Werth et al. Hicks 2018)Wild Type Whole Cell ExtractIYEVSDSEDEELEDDPSERIYEVpSDSEDEELEDDPSER
Cre02.g076900.t1.1--S78 (Werth et al. Hicks 2018)Wild Type Whole Cell ExtractGQPGDGSGPLQQPSPAMPGAKGQPGDGSGPLQQPpSPAMPGAK
Cre02.g076900.t1.1--T859(Werth et al. Hicks 2018) AZD-insensitive Whole Cell ExtractYIGGDKTYTICGTPDYQAPEVIMR
Cre02.g076900.t1.1--S126-S128(Werth et al. Hicks 2018) AZD-insensitive Whole Cell ExtractIYEVSDSEDEELEDDPSER
Cre02.g076900.t1.1--T857(Werth et al. Hicks 2018) AZD-insensitive Whole Cell ExtractTYTICGTPDYQAPEVIMR
Cre02.g076900.t1.1--S71(Werth et al. Hicks 2018) AZD-insensitive Whole Cell ExtractGQPGDGSGPLQQPSPAMPGAK
(Pan et al. Hippler 2011)Purified Flagella AQSINRGQPGDGSGPLQQPSPAMPGAK
(Pan et al. Hippler 2011)Purified Flagella EKIYEVSDSEDEELEDDPSER
(Pan et al. Hippler 2011)Purified Flagella GQPGDGSGPLQQPSPAMPGAK
(Pan et al. Hippler 2011)Purified Flagella IYEVSDSEDEELEDDPSER
ChlamyFP Peptides
Fraction Sequence
(click on peptide to see other models containing this peptide)
B25DLKPENLLLDTQGYIK
B11NAMPGAAAAVEER
B11IIGTGQFGLVR
B11DTGLKNEEAVVAALKK
B10DTGLKNEEAVVAALK
B10MYEDGQPLIR
B10LLAGSIFNDK
B10MADFGFAK
B09GLSDNDLIR
B08QQPQATQQK
© Gregory J. Pazour