The Chlamydomonas Flagellar Proteome Project
ChlamyFPv5 (gene name | annotation): PKL1 | "Similar to Protein Phosphatase"
Aliases: PKL1
Structure: Transition Zone
References: Transition Zone Protein (18 spectral counts [Diener et al. Rosenbaum 2015])
CrFP Total Peptides = 3 (0 Axonemal Peptides | 3 M+M Peptides | 0 KCl Peptides | 0 Tergitol Peptides)
Wang Peptides (Wang et al. Pan 2017) = (enriched fold by ciliary shortening)
Jordan Peptides (Jordan et al. Pigino 2018) = Wild Type M+M: 12 | Dhc1b-3 M+M: 15 | Ift139-2 M+M: 17 (Total Unique Peptides)
Phytozome (gene name | defline): | Protein Phosphatase 1 beta
Annotation v5.6 Symbol | Defline: PKL1 | Protein Phosphatase 1 beta
Annotation v5.6 Description: Serine/threonine protein phosphatase 1 Beta (PP1b); has a Kelch repeat at the N-terminus; found in the flagellar proteome [PMID: 15998802]. Similar to Arabidopsis BSL phosphatases. ts-lethal mutations block in G2 (PMID 25336509)
Annotation v5.6 Synonyms: Cre01.g050850.t1.1#PKL1#g1126.t2
Steve King Dynein Anotation: |
Yuqing Hou's Human Homologs: , , (Blast E value: | Reprocal Best Match? )
Albee et al. Dutcher 2013 Human Homolog: PPP1CB, serine/threonine-protein phosphatase PP1-beta catalytic subunit
Lipid Modifications (N-Myristoylation: MGXXXS/T, S-Geranylgeranylation: CAAX , S-Geranylgeranylation: CC/CXC, S-Farnesylation: CAAX predicted by GPS-Lipid):
Fold Upregulated Post Deflagellation (Albee et al. Dutcher 2013) at 3 min: 0.87; at 10 min: 5.20; at 30 min: 1.73; at 60 min: 3.10
Importance of XAP5 for expression (Li et al. Hu 2018) Wild Type: FPKM | XAP Mutant: FPKM | Fold Change:
CLIP Library: Cre01.g050850
Phytozome Transcript: Cre01.g050850.t1.2
PhosphoPeptides
Protein Reference Source Peptide pLOGO
Cre01.g050850.t1.2--S485(Werth et al. Hicks 2018) AZD-insensitive Whole Cell ExtractQLSIDQLDNEGR
Cre01.g050850.t1.2--S446(Werth et al. Hicks 2018) AZD-insensitive Whole Cell ExtractSSSPDVSLGKDHIPGTTPYTPDVK
Cre01.g050850.t1.2--S442(Werth et al. Hicks 2018) AZD-insensitive Whole Cell ExtractSSSPDVSLGK
Cre01.g050850.t1.2--S512(Werth et al. Hicks 2018) AZD-insensitive Whole Cell ExtractEKSPLFTR
(Pan et al. Hippler 2011)Purified Flagella SSSPDVSLGKDHIPGTTPYTPDVK
(Pan et al. Hippler 2011)Purified Flagella SSSPDVSLGKDHIPGTTPYTPDVK
(Pan et al. Hippler 2011)Purified Flagella SSSPDVSLGKDHIPGTTPYTPDVK
ChlamyFP Peptides
Fraction Sequence
(click on peptide to see other models containing this peptide)
B12AAAEEAAAAAALNVR
B12TSDSPSTNR
B12VLVSGPGPSAR
© Gregory J. Pazour